2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster

Taís Mayumi Kuniyoshi; Andrés González; Sara López-Gomollón; Ana Valladares; M. Teresa Bes; Marı́a F. Fillat; M. Luisa Peleato

doi:10.1016/j.febslet.2011.10.034

November 2, 2011FEBS LettersOpen Access

2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster

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Key Points

This research investigates how 2-oxoglutarate influences NtcA binding to microcystin gene promoters in Microcystis aeruginosa.
Utilized band-shift assay (EMSA) to analyze NtcA binding to promoter regions of microcystin gene cluster.
Examined the effect of varying concentrations of 2-oxoglutarate on NtcA binding affinity at physiological levels.
Assessed binding affinities for fragments of the mcyDA, mcyE, and mcyH promoters.
NtcA binding affinity to the mcyA promoter region increased by 2.5-fold in the presence of 2-oxoglutarate.
The optimal effect of 2-oxoglutarate was observed at a concentration of 0.8 mM, correlating with nitrogen-limiting conditions.
Findings indicate that 2-oxoglutarate acts as a regulatory signal for the microcystin gene cluster under varying nitrogen levels.

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Implication

Band-shift assay reveals 2-oxoglutarate enhances NtcA activity on microcystin genes, suggesting regulatory role in nitrogen balance.

Abstract

The binding affinity of NtcA towards promoter regions of the microcystin gene cluster from Microcystis aeruginosa PCC 7806 has been analyzed by band-shift assay (EMSA). The key nitrogen transcriptional regulator exhibits affinity for two fragments of the bidirectional mcyDA promoter, as well as for promoter regions of mcyE and mcyH. The presence of 2-oxoglutarate increased by 2.5 fold the affinity of NtcA for the mcyA promoter region. The 2-oxoglutarate effect peaked at 0.8 mM, a physiological concentration for this compound under nitrogen-limiting conditions. The results suggest that the 2-oxoglutarate level, as a signal of the C to N balance of the cells, regulates the microcystin gene cluster.

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Kuniyoshi et al. (2011) studied this question.

synapsesocial.com/papers/6a6e98b178a11c550e08886d https://doi.org/10.1016/j.febslet.2011.10.034

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2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster

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Cite This Study

2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster

Key Points

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Implication

Abstract

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Cite This Study

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