Key result
ANFR-A, -B, and -C mRNA are expressed in lactotrophs, corticotrophs, and gonadotrophs of the rat anterior pituitary, with ANFR-A and -B amounts twice that of ANFR-C.
The study demonstrates that ANF receptor subtypes A, B, and C are expressed in specific cell types of the rat anterior pituitary, suggesting it is an important target tissue for ANF action.
Rat pituitary ANFR expression suggests neuroendocrine ANF targets; leaves open human cardiovascular-endocrine relevance.
Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor-A, -B and -C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was detected by in situ hybridization. The amounts of ANFR-A and -B mRNA were found to be similar, and to be twice that of ANFR-C mRNA. At the ultrastructural level, the three types of ANFR mRNA were expressed in three anterior pituitary cell types, namely lactotrophs, corticotrophs, and gonadotrophs, identified by their hormonal content. No signal was revealed in somatotrophs or thyrotrophs. The different forms of mRNA were similar in terms of subcellular localization: in the cytoplasmic matrix and the nuclear euchromatin. These data indicate that the anterior pituitary is an important target tissue for ANF action.
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Grandclément et al. (1995) studied this question. ANFR-A, -B, and -C mRNA are expressed in lactotrophs, corticotrophs, and gonadotrophs of the rat anterior pituitary, with ANFR-A and -B amounts twice that of ANFR-C.
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