Randomized trial examines improved l-arginine production in E. coli using metabolic engineering and strategic feeding, indicating a new fermentation strategy.
Key Points
The aim is to enhance l-arginine production in E. coli by identifying and eliminating metabolic bypasses related to acetyl phosphate.
Engineered E. coli for l-arginine production and identified metabolic bypasses.
Deleted ptsG to reduce overflow metabolism and applied glucose-glycerol co-feeding strategy for improved growth.
Used LC-MS and in vitro assays to analyze byproduct formation and elucidate mechanisms of accumulation.
Achieved l-arginine titer of 65.13 g/L with 47.7% conversion yield in a 5-L bioreactor.
Deleted eutD significantly suppressed byproduct N δ-acetylornithine accumulation while optimizing main pathway flux.
Revealed that high carbon flux and enzyme promiscuity create hidden byproduct routes affecting yield.