Randomized trial identifies a sperm-specific isoform of cAMP-dependent protein kinase in Mytilus galloprovincialis, suggesting unique regulatory roles in activation.
Key Points
This study aims to investigate the expression of a specific isoform of cAMP-dependent protein kinase in mussel spermatozoa.
Purification of the protein from male gonadal tissue.
Identification using LC-MS/MS and sequence analysis.
Immunolocalization to determine distribution in spermatozoa.
The identified isoform is specifically expressed in mussel spermatozoa.
Structural analysis shows unique N-terminal region differences from mammalian counterparts.
Phosphoproteomic analysis identifies two phosphorylation sites exclusive to the mussel isoform.