Binding of NADH and quinoid dihydropteridine to dihydropteridine reductase from rat liver has been studied by the techniques of protein fluorescence quenching and ligand protection. The dissociation constans obtained for the binary complexes by the two methods were in agreement. For NADH, the constant was 0.07 μM and for quinoid dihydropteridine, 2–3 μM (30 °C, pH 6.8). The constants have been compared with the apparent Michaelis constants.
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K.E. Lind (1973) studied this question.
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