A clinical assessment of portable point-of-care testing for quick cortisol assay during adrenal vein sampling

Figures

• 

  Figure 1. The scatter plot of cortisol concentration (QCA versus routine method). Of the adopted sites of cortisol concentration measurement, samples that were lower than the upper limit of the QCA and routine method were plotted (n = 330). The X-axis represents the value of cortisol concentration measured by the routine method. The Y-axis represents the value of cortisol concentration measured by the QCA. The red line represents the regression line.

• 

  Figure 2. The Bland–Altman plot of the cortisol concentration (QCA versus routine method). Of the adopted sites of cortisol concentration measurement, samples that were lower than the upper limit of the QCA and routine method were used to plot the Bland–Altman plot (n = 330). The purple/red line represents a 95% confidence interval. The green line represents the mean value.

• 

  Figure 3. The scatter plot of SI (QCA versus routine method). Of the adopted sites of cortisol concentration measurement, samples that were lower than the upper limit of the QCA and routine method were used to calculate the SI and plot the scatter plot of the SI (n = 242). The X-axis represents the value of SI computed by measurements from the routine method. The Y-axis represents the value of SI computed by measurements from the QCA. The red line represents the regression line.

• 

  Figure 4. The Bland–Altman plot of the SI (QCA versus routine method). Of the adopted sites of cortisol concentration measurement, samples that were lower than the upper limit of the QCA and routine method were used to calculate the SI and were used to plot the Bland–Altman plot (n = 242). The purple/red line represents a 95% confidence interval. The Green line represents the mean value.

• 

  Figure 5. The turnaround time of the AVS. The turnaround time was defined as the time for the first right adrenal vein samples to determine AVS success or failure after taking IVC samples. The histogram above shows the distribution of the turnaround time. A box plot of the turnaround time was drawn below the histogram.

• 

  Figure 6. The outlines of the QCA and immunochromato reader (A, B). (A) The test plate and the internal structure of the QCA. Competitive antigens and anti-cortisol antibodies are arrayed at the immunochromatographic paper’s test and control lines, respectively. Gold nanoparticle labelled anti-cortisol antibodies are fixed in the drip site. (B) Immunochromato reader. The CMOS camera measures the brightness of the reflection from the gold nanoparticles on the test line of the test plate using the light source. The control circuit regulates the output of the CMOS camera.