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Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. These resonances a r e well resolved, allowing identification a n d quantitative analysis of phospholipids in a mixture. T h e chemical shift depends strongly on p H , reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents. -London, E., and G. W. Feigenson. Phosphorus NMR analysis of phospholipids in detergents. J . Lipid Re.\.
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London et al. (1979) studied this question.
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