A new assay method for thyrotropic hormone (TSH) based on the in vitroiodination of thyroglobulin, is presented. After incubation of hog thyroid slices for 15mins. in a modified Krebs-Ringer phosphate buffer containing 0.05μgI/ml of iodide labeled with I131 and TSH, the radio-activity in the protein fraction of slices was measured and utilized as an index of TSH activity. The usable range of this assay was 1 I.mU./ml to 30 I.mU./ml of TSH and the index of precision varied from 0.15 to 0.30. Effects of temperature, pH and constituents of the medium upon the response were studied.
No takes yet. Share an insight, caveat, or question.
Kondo et al. (1963) studied this question.