Isolation and Characterization of Arsenic Resistance Bacteria from Groundwater Sample

Arsenic is a kind of metalloid that is deemed detrimental because of its carcinogenic and toxic properties. The presence of arsenic in drinking water and soil endangers more than 100 million people worldwide. Since biological agents can be represented as an economical substitution to expensive metal decontamination technologies, the major purpose of this work was to identify and isolate microorganisms that are resistant to arsenic and can remove it from the environment. Seven arsenic resistant and arsenite-oxidizing bacteria were recovered from the arsenic contaminated groundwater from Chapainawabganj, Bangladesh. These strains were closely linked to several species of Acinetobacter, Serratia, Corynebacterium, Klebsiella and Staphylococcus based on their investigated morphology and biochemical features. Between 300 and 1600 mM, the isolates' minimum inhibitory concentrations (MIC) on arsenite varied widely which suggested the recommendation of a strong potential to be employed in bioremediation of arsenic. Moreover, as all these isolates displayed arsenite oxidation to arsenate, it revealed the existence of arsenite oxidase enzyme which would open a new possibility of bioconversion of arsenic into a less dangerous form. Antimicrobial investigation indicated that Chloramphenicol, Cefepime, Erythromycin, Cefotaxime, Gentamicin, Ceftriaxone and Colistin had extremely susceptible rates while Ceftazidime, Amoxicillin and Ampicillin had high to moderate resistance. The study stated that the isolated strains may simply be employed as a novel microorganism for bioremediation of soil, water and effluents and the enzymes produced may be exploited for industrial operation, though further research should be done.