Pharmacological Investigation of Ranger Capsule by Studying Acute Toxicity, in Vitro Anti Oxidant Property and in Vivo Anti-Stress Activity

The stress is generally considered as the functional adaptation of the organism in order to cope up with changing and challenging environment. Exposure to stressful conditions is among the most common human experience. It has been reported that exposure to physical and mental stress situations can stimulate numerous pathways, leading to increased production of oxygen free radicals. In such situation, the antioxidant defense systems of body may become insufficient to scavenge all free radicals. In the present study, an attempt was made to evaluate acute toxicity of a herbo-mineral Ayurvedic formulation, Ranger capsule along with its in-vitro anti-oxidant property and in-vivo anti-stress activity. Acute toxicity study was carried out on wistar rats as per OECD Guidelines 423. For anti-stress activity forced swimming endurance test was carried out on wistar rats. During this study, Difference in rectal temperature and swimming time was noted. Blood samples were collected for estimation of serum cortisol and serum glucose level. The reduction potential of Ranger Capsule was determined by the invitro ferric reducing anti-oxidant power (FRAP) assay at various concentration. Study data revealed that Ranger Capsule didn't produced any toxic effect even up to the dose level of 5000 mg/kg hence was proven as safe. The pre-treatment of Ranger Capsule prevented the fall of rectal temperature in highly significant manner and increased swimming time in comparison to stress control group. Ranger Capsule treated animals showed significant restriction in elevation of serum cortisol and serum glucose level as compared to stress control group. From the available results, it can be concluded that pre-treatment of Ranger Capsule is safe, capable to increase tolerance against non-specific stress in experimental animals and having significant anti-oxidant activity.