A simple color test, capable of detecting pasteurized bottled milk having poor keeping quality under refrigeration, is described. Neotetrazolium dye is mixed with milk in a test tube. The tube is evacuated and sealed, placed in an incubator at 37° C. and examined after four hours. A detectable pink color appearing in the sample is an indication that the milk will undergo flavor spoilage within a four day period. This test apparently measures the psychrophilic activity in the milk.
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Day et al. (1956) studied this question.
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