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A chemically defined medium capable of supporting the growth of pneumo-coccus would be of value in studies on the metabolism and sulfonamide resistance of strains of this organism and would simplify the isolation of metabolic products. Before a medium may be considered to satisfy the nutritional requirements of a microorganism it must meet two qualifications-it must permit a heavy growth of the organism starting with a reasonably small inoculum, and it must permit unlimited subculture of the organism. Rane and Subbarow (1940) have developed a gelatine hydrolyzate medium which supported growth of four strains of pneumococcus, but failed to permit growth of a fifth strain, and no report of subculture on this medium was made. Cohen, Halbert, and Perkins (1942) reported poor growth and "failure of growth in successive transplants " on the medium of Rane and Subbarow. In our own experience none of ten strains tried would grow on subculture in this medium. Rane and Subbarow demonstrated the essentiality of pantothenic acid, nicotinic acid, and choline for the strains they tested. A mixture of known amino acids
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Adams et al. (1945) studied this question.
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