Answers are grounded in this paper and cite their sources.
Unconjugated bilirubin is highly soluble in formamide with cyanide (0.10 mol/1), giving a solution stable at 25 °C in the dark for 4 hours. Human serum (at pH = 9.5) can be enriched with this solution to at least 430 μmol/1 (250 mg/1) even when the volume fraction of formamide is below 0.02. The bilirubin complex in the ensuing preparation (employing infant serum) seems to have the same stability and spectrophotometric properties as in serum from hyperbilirubinaemic infants. This reference preparation is suitable for standardization of methods using azobilirubin or direct spectro-photometry for the determination of total bilirubin in human serum.
No takes yet. Share an insight, caveat, or question.
Dybkær et al. (1970) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: