Key result
Nicotine and its metabolites increased both the activity and expression of angiotensin-converting enzyme in human endothelial cells, with marked individual variation.
Why the study?
Do nicotine and its metabolites increase ACE activity and expression in human endothelial cells?
Do nicotine and its metabolites increase ACE activity and expression in human endothelial cells?
Nicotine and its metabolites increase ACE activity and expression in human endothelial cells, suggesting a cellular mechanism for smoking-induced vascular damage.
May link nicotine to endothelial ACE upregulation; hypothesis-generating and requires in vivo confirmation before clinical consideration.
Nicotine has been shown to induce endothelial dysfunction, which is an early marker of atherosclerosis. Nicotine undergoes extensive metabolism in the liver, forming a number of major and minor metabolites. There are very limited data on the effect of nicotine metabolites on the cardiovascular system. This study investigates the effects of nicotine and the nicotine metabolites, cotinine, cotinine-N-oxide, nicotine-1'-N-oxide, norcotinine, trans-3'-hydroxycotinine, on angiotensin-converting enzyme (ACE) in human endothelial cells. Cultured endothelial cells obtained from human umbilical cord vein (HUVECs) were stimulated with nicotine or nicotine metabolites in concentrations similar to those observed in plasma during smoking. ACE activity and expression were analyzed using commercial kits. The results showed that nicotine and nicotine metabolites can increase both activity and expression of ACE. However, a marked individual variation in the response to the drugs was observed. This variation was not associated with the ACE insertion/deletion polymorphism. Tobacco contains numerous chemical compounds, and the underlying cause for development of atherosclerosis in smokers is probably multifactorial. The results from this study could explain one cellular mechanism by which smoking exerts negative effect on the vascular system.
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Ljungberg et al. (2008) studied this question. Nicotine and nicotine metabolites was evaluated on Angiotensin-converting enzyme (ACE) activity and expression. Nicotine and its metabolites increased both the activity and expression of angiotensin-converting enzyme in human endothelial cells, with marked individual variation.
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