January 1, 1975Journal of General Virology

Determination of the Molecular Weight of Bovine Enterovirus RNA by Nuclease Digestion

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Population

[32P]-labelled bovine enterovirus RNA

Design

Preclinical

Authors

DTD. ToddUniversity of North Carolina at Greensboro SMSamuel MartinEuropean Bioinformatics Institute

Discussion

Member takes

Implication

Provides baseline bovine enterovirus RNA size; leaves open further molecular and translational virology studies.

Structured PICO

Population

[32P]-labelled bovine enterovirus RNA

Intervention

Digestion with pancreatic RNase and separation of oligonucleotides using two-stage fractionation on DEAE-Sephadex in 7 M-urea at pH 7-6 and pH 3-0

Outcome

Molecular weight of the RNAsurrogate

The molecular weight of bovine enterovirus RNA was determined to be 2.93 ± 0.02 x 10^6 using nuclease digestion, agreeing with sedimentation and gel electrophoresis estimates.

Cite This Study

Todd et al. (1975) studied this question.

synapsesocial.com/papers/6a6f57d2ac440176ef2808f6 https://doi.org/10.1099/0022-1317-26-1-121

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