Persistent circulation of Coxsackievirus A6 of genotype D3 in mainland of China between 2008 and 2015

Figure 1. Geographic and annual distribution of 807 Chinese CV-A6 strains included in this study.

Figure 2. Phylogenetic trees based on the complete VP1 nucleotide sequences of CV-A6 of 65 representative strains isolated between 1949 (the prototype Gdula strain) and 2015. Numbers at the nodes indicate bootstrap support for each node (percentage of 1000 bootstrap replicates). A difference of at least 15% in the entire VP1 region of CV-A6 strains was used to distinguish genotypes. CV-A6 strains are labelled using the following format: ‘isolate name’/‘country of origin’/‘year of isolation’ (details are listed in Table 1). Different genotypes and sub-genotypes are shown to the right of the tree and the scale at the bottom indicates measurement of relative phylogenetic distance. Abbreviations of countries/Province: CHN, China; JPN, Japan; IND, India; TW, Taiwan, China; FIN, Finland; FRA, France; ESP, Spain.

Figure 3. Phylogenetic analyses based on 807 entire VP1 sequences of CV-A6 in China. The phylogenetic tree shows the evolutionary tendency of sub-genotype D2 and D3 strains in China since 2008; sub-genotype D3 was the predominant one. The branches of sequences from mainland of China are highlighted in different colours according to years from 2008 to 2015, the other international strains (including Taiwan strains) and strains detected before 2008 are uncoloured. The phylogenetic tree indicates that evolutionary branch D3a was responsible for the recent outbreaks since 2013. A ladder-like topology revealed that adaptation of CV-A6 occurred during the epidemic history. A prototype CV-A 2 strain (Fleetwood) served as an outgroup.

Figure 4. Yearly proportional distribution of HFMD-associated sub-genotypes D2 and D3 (evolutionary branches D3a and D3b) from 2008 to 2015 in mainland of China.

Figure 5. The geographic distribution of HFMD-associated CV-A6 strains in 7 representative regions of China, the region and the number of the sequences are coloured according to the legend (Taiwan sequences are not included). The free map source was acquired from d-maps.com (URL: http://d-maps.com/carte.php?num_car=15272&lang=en; the content is properly attributed in the appropriate figure legend according to the attribution guidelines; the website is protected by Copyright France, Registration Number: 58KU297). The map was then generated and modified using Microsoft Excel 2010 software.