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In situ hybridization is a valuable technique for detecting and visualizing specific viral nucleic acids, such as Epstein-Barr virus, within individual cells in formalin-fixed, paraffin-embedded tissues.
This review outlines the technical approaches and applications of in situ hybridization for detecting viral nucleic acids in tissue samples.
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May inform archived tissue viral diagnostics; leaves open prospective validation before routine adoption.
In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.
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Mohamed Mabruk (2004) conducted a review in Viral infection (Epstein-Barr virus). In situ hybridization was evaluated. In situ hybridization is a valuable technique for detecting and visualizing specific viral nucleic acids, such as Epstein-Barr virus, within individual cells in formalin-fixed, paraffin-embedded tissues.
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