The inhibitory effects of fourteen kinds of 12-13-epoxy trichothec mycotoxins and related antibiotics on protein and DNA syntheses in reticulocytes, Ehrlich ascites tumor cells, rat liver and bacteria were investigated in whole-cell and cell-free systems, and the following results were obtained; 1. Among the fourteen compounds tested, twelve trichothecenes inhibited the uptake of 14C-leucine by whole cells of rabbit reticulocytes. The inhibitory effect varied over a wide range on chemical modification of the side chain and double bond. 2. In Ehrlich ascites tumor cells, the mycotoxins inhibited the uptake of 14C-leucine and 14C-thymidine without affecting that of14C-uracil. 3. When rabbit reticulocytes were treated with low concentrations of the trichothecenes, a marked degradation of polyribosomes was observed. 4. In cell-free systems of reticulocytes and rat liver, the trichothecenes inhibited the poly-U-dependent synthesis of polyphenylalanine in concentrations lower than the inhibitory concentrations of puromycin and cycloheximide, and the concentrations of various trichothecenes required for 50% inhibition were correlated with their lethal doses in mice. 5. In whole-cell and cell-free systems of Escherichia coli, fusarenon-X did not inhibit protein synthesis. A high concentration of the mycotoxin, slightly inhibited the uptake of 14C-amino acid in yeast (Geolrichum candidum). 6. Biochemical features of the actions of the trichothec mycotoxins were discussed in relation to their chemical and toxicological characteristics.
No takes yet. Share an insight, caveat, or question.
Ueno et al. (1973) studied this question.