The ferric ammonium sulfate and modified vanillin‐hydrochloric acid methods of tannin analysis were used to measure the tannin content of samples of sorghum [ Sorghum bicolor (L.) Moench] grain. The overall correlation between the two methods was good when all samples were considered. Samples of ‘Ga 615,’ a brown‐seeded hybrid, were grown at each of six locations for 3 years. Both methods of tannin analysis showed a significant environmental effect on tannin content. The genes, B 1 B 2 S, that control pericarp color and presence or absence of a pigmented testa significantly affected tannin content. The major effect was a high tannin content associated with the pigmented testa. Tannin content was higher in grain with a red pericarp than grain with a white pericarp in both nonrelated material and in near‐isogenic lines. This pericarp pigmentation is controlled by genes other than the B 1 B 2 S genes.
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Maxson et al. (1972) studied this question.