A relatively inexpensive micro-Raman system featuring confocal imaging, holographic optics, and CCD detection is described in detail. The construction of a confocal filter is discussed, along with the depth of field and spatial rejection expected. Limitations of the system such as low-frequency signal rejection by the holographic optics and wavelength calibration are also discussed. Spectra are shown giving examples of the capabilities of the system and the signal-to-noise ratio expected for typical liquid and solid samples.
No takes yet. Share an insight, caveat, or question.
LaPlant et al. (1995) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: