Generation of Fertile Cloned Rats by Regulating Oocyte Activation

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In this study, we achieved a breakthrough in cloning rats using somatic cell nuclear transfer (SCNT), overcoming longstanding challenges associated with the rapid and incomplete activation of rat oocytes following their removal from the oviduct. By employing the protease inhibitor MG132, we were able to stabilize oocytes and prevent premature activation, allowing sufficient time for SCNT to be successfully performed.This refined protocol resulted in the production of viable embryos, which, when implanted into foster mothers, led to the birth of live cloned rats. These clones included individuals that reached maturity and produced healthy offspring, demonstrating the technique’s robustness. Our work establishes a foundation for precise genetic modifications in rats, such as conditional knockouts and gene replacements, making it possible to develop more relevant models for studying human diseases.