A 2-naphthylamine-1-sulfonate (tobias acid)-degrading Pseudomonas strain was isolated from soil. This organism degraded 1-naphthalenesulfonate and 2-naphthol-1-sulforiate as well as tobias acid. When the cells grown on a nutrient medium were incubated with tobias acid, 1-naphthalenesulfonate, and 2-naphthol-1-sulfonate, salicylate was temporarily accumulated in the culture medium. A salicylate-degrading enzyme and a gentisate-degrading enzyme were induced by salicylate and gentisate, respectively. On the other hand, the enzymes which degrade sulfonated naphthalenes to salicylate were constitutive. Ammonia, sulfate, and a small amount of sulfite were detected in the course of degradation of tobias acid. 2-Naphthol-8-sulfonate or 1-naphthol-5-sulfonate was converted into 2,4-dihydroxybenzoate or 2,6-dihydroxybenzoate respectively. Both cis-1,2-dihydroxy-1,2-dihydro-3-naphthalenesulfonate and sulfate were formed when the cells were incubated with 2-naphthalenesulfonate. It is suggested that tobias acid was degraded by 1,2-dioxygenation in the initial process of the degradation pathway.
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Ohe et al. (1990) studied this question.