Cell proliferation in growing cultures of dissociated embryonic mouse brain: macromolecule and ornithine decarboxylase synthesis and regulation by hormones and drugs

Primary cultures of cells dissociated from embryonic mouse brain were demonstrated to be a useful system for studying cell proliferation and its regulation. Ornithine decarboxylase activity was closely correlated with the rate of DNA and RNA synthesis during cell growth, suggesting that the enzyme is as good an indicator of cell proliferation in these cultures as it is in vivo. Both DNA synthesis and ornithine decarboxylase activity were stimulated by insulin. The enzyme was stimulated five- to sixfold by insulin and approximately twofold by butyrate, cis-retinoic acid, and 12-O-tetradecanoylphorbol-13-acetate. No effect on the enzyme activity was observed with triiodothyronine, hydrocortisone, growth hormone, cyclic AMP, or cyclic GMP.