The dynamics of Rhizobium leguminosarum biovar trifolii introduced into soil as determined by immunofluorescence and selective plating techniques

The two genes (cfxP) for phosphoribulokinase (PRK) in Alcaligenes eutrophus H16 are simultaneously expressed, resulting in the formation of PRK isoenzymes. The isoenzymes are structurally and immunoligically closely related. Their subunits differ only slightly in size. Mrs of 33 000 and 32 500 were determined for the chromosomally and megaplasmid pHF1-encoded subunits, respectively. The pHG1-encoded gene, cfxPp, was cloned in Eschirichia coli and expressed under the cloned in Escherichia coli and expressed under the control of the lac promoter of pUC9 vectors. Native PRK with subunits of Mr 32 500 was formed, confirming the identity and functionality of cfxPp. However, the recombinant PRK had a significantly lower specific activity than the authentic enzyme.