The Collection, Storage and Preparation of Viable Cadaver Marrow for Intravenous Use

A procedure for collecting and storing viable cadaver marrow in a form suitable for intravenous use is described. Vertebral bone marrow is removed under sterile conditions within a few hours after death. The marrow cells are screened and suspended after centrifugation in an equal volume of a liquid medium consisting of 30 per cent glycerol and 70 per cent human serum and frozen to -80 C. in plastic bags. Suggestions for thawing, deglycerolizing and administering the cells intravenously are given.