Three methods of antiserum production have been compared. One of these consistently gave rise to greater homologous quellung titres and was used routinely. A modification of this method using vaccines consisting of washed, killed suspensions of type strains resulted in no significant increase in titres or decrease in cross‐reactions. Considerable differences were found between the homologous quellung titres and cross‐reactions of antisera prepared in the same laboratory on different occasions.
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Edmondson et al. (1979) studied this question.
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