THE method for the estimation of calcium in blood-serum devised by Kramer and Tisdall [1921] has been extensively used and, it is generally agreed, gives reproducible results in expert hands.Clark and Collip [1925], however, emphasise that the details of the method must be followed with meticulous accuracy or discrepant results will be obtained.They attribute the main source of error to the slight solubility of calcium oxalate and are of opinion that traces of ammonium oxalate, used for washing the precipitate, remain, and, if close adherence to the original method is maintained, tend to com-
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Trevan et al. (1926) studied this question.
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