The Freeze-Fracture Technique: Application to the Study of Animal Plasma Membranes

During recent years, the freeze-fracture (FF) technique has become one of the most useful procedures available for the ultrastructural analysis of cell components, particularly for the study of biological membranes. The method has gradually evolved from a highly specialized and technically complex procedure to a reasonably accesible one, mainly as a consequence of improvements in commercial FF equipment, the understanding of the fracturing process and the artifacts induced, and the development of ancillary techniques for the study of cell membrane organization. Due to these advances, the FF method can be considered at present as an almost standard procedure for biological electron microscopical laboratories.