The experimental herbicide EMD-IT 5914 [difunon, 5-dimethyl-amino-methylene-2-oxo-4-phenyl-2,5-dihydrofurane-carbonitrile-(3)] was applied to unicellular algae and its effect on growth, oxygen evolution and photosynthetic electron transport measured. Inhibition of biosynthesis of chloroplast pigments was evaluated in relation to the activities of porphobilinogenase and δ-aminolevulinic acid dehydratase. The only direct effect of the herbicide was an inhibition of carotenoid biosynthesis but not of photosynthetic electron transport or enzymic activities connected with porphyrin biosynthesis. Chlorophyll bleaching is considered to be a secondary process.
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Kunert et al. (1978) studied this question.
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