What question did this study set out to answer?

To develop and validate an improved Vaseline gap potentiometric recording and voltage clamp technique tailored for single skeletal muscle fibers.

March 1, 1976The Journal of General PhysiologyOpen Access

An improved vaseline gap voltage clamp for skeletal muscle fibers.

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Authors

BHBertil HilleUniversity of Washington DCD. T. CampbellUniversity of Iowa

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Implication

Experimental study demonstrates accurate voltage clamp recording in frog skeletal muscle fibers, indicating robust surface membrane control despite uncontrolled transverse tubules.

Key Points

To develop and validate an improved Vaseline gap potentiometric recording and voltage clamp technique tailored for single skeletal muscle fibers.
Modified the Frankenhaeuser-Dodge voltage clamp configuration to enhance frequency response and compensate for series resistance and complex current-pathway impedance.
Mounted cut single fiber fragments from frog semitendinosus muscle across Vaseline seals with internal CsF depolarization and central Ringer solution perfusion.
Evaluated measurement errors directly with microelectrodes and simulated passive and active membrane behavior using a disk equivalent circuit of the transverse tubular system.
Peak inward sodium current density reached 3.7 mA/cm² at 5 °C, producing smoothly graded currents without notches to demonstrate effective surface membrane voltage control.
Action potentials were prolonged at 5 °C due to the absence of delayed rectification and matched disk model simulations containing sodium permeability in surface and tubular membranes.
Simulations and recordings showed that while the transverse tubular system exhibits regenerative sodium currents and escapes voltage clamp control, its currents do not produce significant surface recording errors at low temperatures.

Cite This Study

Hille et al. (1976) studied this question.

synapsesocial.com/papers/6a6f86398031ec7bb1dbcd02 https://doi.org/10.1085/jgp.67.3.265

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Implication

Experimental study demonstrates accurate voltage clamp recording in frog skeletal muscle fibers, indicating robust surface membrane control despite uncontrolled transverse tubules.

An improved vaseline gap voltage clamp for skeletal muscle fibers.

Discussion

Member takes

Implication

Key Points

Cite This Study

An improved vaseline gap voltage clamp for skeletal muscle fibers.

Discussion

Member takes

Implication

Key Points

Cite This Study

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