Seasonal phosphatase activities of mosses from Upper Teesdale, northern England

Changes in tissue nutrient concentrations and surface phosphatase activities of eight moss species were measured over one year in terrestrial and semi-aquatic environments on Widdybank Fell, Upper Teesdale National Nature Reserve, northern England. Rates of phosphatase activity in apical regions of moss shoots differed markedly between species, but were generally greatest in the winter and least in the summer in most species. Mean values for phosphomonoesterase activity (µmol para-nitrophenol g-1 d.wt h-1) ranged from 18.2 for Polytrichum commune to 85.8 for Palustriella commutata var. falcata. Mean phosphodiesterase activity ranged from 3.1 for Polytrichum commune to 86.2 for Hylocomium splendens. In contrast, tissue nitrogen and phosphorus concentrations remained relatively constant throughout the year. Phosphatase activities were negatively correlated with tissue phosphorus concentration for several species, although few relationships were detected between ambient nutrient concentrations and phosphatase activity, tissue nitrogen, or tissue phosphorus concentration. These results demonstrate that phosphatase activities can provide a sensitive indicator of nutrient stress in terrestrial and semi-aquatic mosses, notably in the ectohydric Hylocomium splendens. However, further studies at sites with a wide range of nutrient levels are required to determine whether the technique can be used to indicate ambient nutrient status.