Key result
Olive oil peptides lower blood pressure and inhibit ACE activity in hypertensive rats.
The low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698-1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) <10 µm. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR.
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Alcaide‐Hidalgo et al. (2020) studied Hypertension. Synthetic peptides RDGGYCC and CCGNAVPQ (from unfiltered virgin olive oil) was evaluated on Antihypertensive and ACE inhibitory activity. Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ from unfiltered virgin olive oil showed antihypertensive and ACE inhibitory activity in spontaneously hypertensive rats.
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