Poly(vinyl ester resin-co-ethylene dimethacrylate) monolithic columns have been prepared by in situ copolymerization of the monomers in the presence of a porogenic agent. The monolith was further derived by 3-glycidoxypropyltrimethoxysilane and then hydrolyzed in 0.25 mol/L sulfuric acid to prepare diol column. The chicken egg white (CEW) was separated by a column with a spacer arm and another without a spacer arm. The results showed that the derived monolith had a higher separation capacity. Moreover, the separations under different flow rates were compared. The separation could be carried out at the flow rate of 3.0 mL/min, and one separation takes place within 3 min. Finally, the maximum amount of adsorption of lysozyme on the monolith was obtained by the use of a pH and concentration gradient.
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Liu et al. (2010) studied this question.
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