Extrazelluläre Lipase aus Acinetobacter calcoaceticus

Cell‐free growth liquor of Acinetobacter calcoaceticus 69‐V contains an extracellular lipase. Its activity depends on growth phase and carbon source. During growth on acetate or succinate the activity ist low or zero, respectively. Growth on alkanes causes an increase in the extracellular lipase activity. Activity reaches maximum values during the exponential phase of growth which significantly decrease in the stationary phase During the growth on alkanes some surfactants (Tauroglycocholate, Triton X‐405) stimulate the excretion of the enzyme and some other (Tween, Brij, Triton X‐100) inhibit the lipase and growth of cells, respectively Air‐water and alkane‐water interfaces inhibit the lipase activity. During starvation of the bacteria grown on alkanes lipase is excreted in the starvation medium.