Authenticity assessment of trans -anethole is deduced from 2 H/ 1 H and 13 C/ 12 C isotope ratios, determined by gas chromatography−isotope ratio mass spectrometry (GC−IRMS). For that purpose, self-prepared anise and fennel oils, and synthetic and “natural” samples of trans -anethole, as well as commercially available anise and fennel oils have been investigated. Authenticity ranges of 2 H/ 1 H and 13 C/ 12 C isotope ratios of trans -anethole were defined. Scope and limitations of the applied online GC−IRMS techniques are discussed.
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Bilke et al. (2002) studied this question.
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