Simultaneous analysis of hydrochlorothiazide, triamterene, furosemide, and spironolactone by densitometric TLC

A new chromatographic and densitometric method has been established for identification and quantitative analysis of hydrochlorothiazide, triamterene, furosemide, and spironolactone, which are present, together, in complex drugs used to treat hypertension. For separation, silica gel F 254 plates were used with hexane-ethyl acetate-methanol-water-acetic acid 8.4:8:3:0.4:0.2 (v/v) as mobile phase. Densitometric measurements were performed at 264 nm selected for all of the constituents. The method is specific for the analyte constituents examined, and characterized by high sensitivity; LOD is from 0.022 to 0.150 μg per band, LOQ from 0.068 to 0.450 μg per band, recovery from 97.10 to 101.02%, and linear range from 0.060 to 2.650 μg per band. The method is characterized by good precision with RSD from 0.66 to 0.96%.