Section I: Introduction and metabolism Section II: Sampling and extraction Section III: Physio-chemical methods Section IV: Pharmacological and immunoassays Section V: Enzymology and lipid peroxidation Section VI: Related techniques Appendix: Eicosanoids: structure, availability, storage and stability Abbreviations Introduction to the eicosanoids Metabolism of prostaglandins and lipoxygenase products: relevance for eicosanoid assay Tissue sampling and preparation Extraction of eicosanoids from biological samples Thin-layer chromatography (including radio thin-layer chromatography and autoradiography of prostaglandins and related compounds High pressure liquid chromatography in the analysis of arachidonic acid metabolites Gas chromatography and mass spectrometry of eicosanoids Measurement of prostaglandins, thromboxanes and leukotrienes by smooth muscle bioassay Aggregometry techniques for prostanoid study and evaluation Radioimmunoassay of eicosanoids Enzyme immunoassay Cyclo-oxygenases: measurement, purification and properties Lipoxygenases: measurement, characterization and properties Lipid peroxidation Quantitative measurement of arachidonic acid in tissues or fluids Methods in prostanoid receptor classification Paf-acether (platelet-activating factor) Appendix Index
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A 1989 study studied this question.