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January 1, 2010Analytical Methods

Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by HPLC coupled to ICP-MS

Petru Jitaru Giulio Cozzi Roberta Seraglia Pietro Traldi Paolo Cescon Carlo Barbante

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Abstract

This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid-phase extraction for the accurate determination of glutathione peroxidase (GPx), selenoprotein P (SelP) and selenoalbumin (SeAlb) in human serum by affinity HPLC (AF-HPLC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS). In addition, the identification and the purity assessment of the GPx, SelP and SeAlb peak fractions separated by AF-HPLC is addressed by their analysis using matrix assisted laser desorption ionization-time of flight mass spectrometry.

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Jitaru et al. (2010) studied this question.

synapsesocial.com/papers/6a6fb845abc331a8580790de https://doi.org/10.1039/c0ay00173b

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