The technical and clinical usefulness of three substrates for serum cholinesterase were compared. The diagnostic value of acetylthiocholine was equal to that of butyrylthiocholine and superior to that of o-nitrophenylbutyrate. Technically, butyrylthiocholine is superior to the other two substrates chiefly because of its greater affinity for serum cholinesterase. A kinetic method using o-nitrophenylbutyrate is described that is capable of carrying out 60 tests per hour with good results.
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G Szász (1968) studied this question.
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