A semiautomated high-throughput system has been developed to express and purify proteins and assay their catalytic activity. The screen can be used to evolve activity, selectivity, and expression levels of proteins directly or in combination with selections. To illustrate its potential, the system was applied to the directed evolution of catalytic antibodies with aldolase activity.
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Gildersleeve et al. (2003) studied this question.
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