Fractionation of Plasma Lipoproteins with Preparative Zonal Ultracentrifugation

An improved technique has been developed for the separation and isolation of plasma lipoproteins with zonal Ultracentrifugation. The lipoproteins of 25 ml plasma were fractionated by flotation in a MSE type B-XV titanium rotor at 120,000 X g for 18 hours using a NaCl-KBr density gradient from d = 1.005 to d = 1.23. Very low density, low density and high density lipoproteins were separated from each other and from the other plasma proteins, and the high density lipoproteins were divided into two subfractions.