SUMMARY.— The effects of various factors on the histochemical demonstration of 3β‐hydroxysteroid dehydrogenase activity in human skin were examined by a semiquantitative technique of granule counting. The optimum concentration of DHA was found to be 0·1 mg./ml. and of NAD 0·3 mg./ml. Vitamin K and nicotinamide enhanced the histochemical demonstration of the enzyme whereas cyproterone and cyproterone acetate inhibited the reaction.
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Muir et al. (1968) studied this question.
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