The construction of a multiplex system for recording the resonance Raman (RR) spectra of enzyme‐substrate complexes in the near‐UV region is described. The instrument is based on commercially available components consisting of a 2 W UV krypton ion laser, a triple spectrograph (in which the first two monochromators act as a zero dispersion, variable bandpass filter) and an intensified diode array. The performance of this system is compared with that of a double monochromator with a photomultiplier as a detector. The enzyme‐substrate systems chosen as examples for the comparison offer a difficult experimental challenge because they evolve rapidly and have poor optical homogeneity. In addition, a simple apparatus is described for recording the RR spectra of enzyme‐substrate complexes in cryosolvents at temperatures as low as −50 °C.
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Carey et al. (1983) studied this question.
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