Single Cell Profiling in the Sox10 Hirschsprung Mouse Implicates Hox Genes in Enteric Neuron Trajectory Allocation

BACKGROUND & AIMS: effects in early ENS development. METHODS: littermates for single-cell RNA sequencing (scRNA-seq). scRNA-seq data was processed to identify cell type-specific markers, differentially expressed genes, cell fate trajectories, and gene regulatory network activity between genotypes. Hybridization chain reaction (HCR) coupled with immunohistochemistry validated expression changes. RESULTS: ENS. CONCLUSIONS: scRNA-seq profiles. This work is the first to correlate changes in Hox expression, notably Hoxa6, with alterations in enteric neuron trajectories.