Nucleoside und Nucleotide Teil 27 Festphasensynthese eines Tridecanucleosid‐dodecaphosphats, das die unnatürliche Base 4‐Amino‐2(1H)‐pyridinon enthält, und eines Octanucleosid‐heptaphosphats zur Untersuchung der DNA‐Polymerase‐Spezifität

Nucleosides and Nucleotides. Solid Phase Synthesis of a Tridecanucleoside Dodecaphosphate Containing the Unnatural Base 4‐Amino‐2(1 H )‐pyridinone and of a Octanucleoside Heptaphosphate for the Determination of the DNA‐Polymerase Specificity The tridecanucleoside dodecaphosphate d (T‐T‐Z‐C‐G‐T‐C‐A‐A‐A‐A‐T‐C) incorporating the modified nucleoside 4‐amino‐1‐(2′‐deoxy‐β‐D‐ribofuranosyl)‐2(1 H )‐pyridinone (Z d , 1 ) and the octanucleoside heptaphosphate d (G‐A‐T‐T‐T‐T‐G‐A) were synthesized by the solid‐phase method using the triester method. The carrier material was a copolymer consisting of styrene with 1% divinylbenzene.