ELMO Recruits Actin Cross-linking Family 7 (ACF7) at the Cell Membrane for Microtubule Capture and Stabilization of Cellular Protrusions

ELMO and DOCK180 proteins form an evolutionarily conserved module controlling Rac GTPase signaling during cell migration, phagocytosis, and myoblast fusion. Here, we identified the microtubule and actin-binding spectraplakin ACF7 as a novel ELMO-interacting partner. A C-terminal polyproline segment in ELMO and the last spectrin repeat of ACF7 mediate a direct interaction between these proteins. Co-expression of ELMO1 with ACF7 promoted the formation of long membrane protrusions during integrin-mediated cell spreading. Quantification of membrane dynamics established that coupling of ELMO and ACF7 increases the persistence of the protruding activity. Mechanistically, we uncovered a role for ELMO in the recruitment of ACF7 to the membrane to promote microtubule capture and stability. Functionally, these effects of ELMO and ACF7 on cytoskeletal dynamics required the Rac GEF DOCK180. In conclusion, our findings support a role for ELMO in protrusion stability by acting at the interface between the actin cytoskeleton and the microtubule network. Background: ELMO and DOCK180 synergize to promote Rac-dependent cell migration. Results: ELMO synergize with a novel interacting protein, ACF7, to promote cytoskeletal changes. Conclusion: ELMO recruits ACF7 at the membrane and impacts on the microtubule network to increase the persistence of membrane protrusion. Significance: ELMO controls protrusion stability by acting at the interface between the actin cytoskeleton and the microtubule network.