The distinction between apomictic nucellar and zygotic 5-month-old seedlings of Citrus was made using two genetically defined isozyme markers: phosphoglucose isomerase and phosphoglucose mutase. Of 123 seedlings of attempted crosses of ‘King’ (seed parent) × ‘Parsons Special’, 18 had the ‘King’ genotype and were therefore nucellars. Segregation ratios for both markers were as expected among the zygotics.
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Soost et al. (1980) studied this question.