SUMMARY: Samples of purified vaccinia virus chemically analysed had the following composition (%): N, 14.7; P, 0.49; S, 0.76; DNA, 3.2; total lipid, 5.0 (including cholesterol, 1.2; phospholipid, 2.1); trace constituents detected were: RNA, 0.1; carbohydrate, 0.2; Cu, 0.02; flavin, 5 × 10−4; biotin, 1.3 × 10−5. These results are compared with other published figures and the differences discussed. The trace constituents are not considered to be true components of the virus. On the other hand, there is no evidence for regarding cholesterol and the other lipid components as non-essential constituents of the virus.
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H. T. Zwartouw (1964) studied this question.
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