Staphylococcal Transfer Ribonucleic Acids

The isoaccepting species of tRNAGly from Staphylococcus epidermidis Texas 26 have been purified. tRNAGlyi the species which fails to participate in protein synthesis but does function in peptidoglycan synthesis, is actually a mixture of two species, tRNAGlyia and tRNAGlyib. tRNAGlyia, tRNAGlyii, and tRNAGlyiii could be purified to homogeneity, whereas tRNAGlyib was obtained only as a mixture with tRNAGlyia. The purification utilized standard column chromatographic procedures for tRNAiGly and tRNAGlyiii, whereas tRNAGlyii was obtained by means of the Tener procedure. Methods were developed for preparing each isoaccepting species labeled with 32P.