Congenital tremor and splay leg in piglets – insights into the virome, local cytokine response, and histology

Figures

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  Fig. 1. Flow chart showing the number of sequenced samples. A total number of 21 piglets were subjected to sequencing; eight healthy, six with signs of congenital tremor, and seven piglets with signs of splay leg. The piglets were selected to represent all sampled farms and litters

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  Fig. 2. Cerebellar tissue from a piglet with signs of congenital tremor and a healthy control piglet. A and B: HE-stained cerebellar tissue from a piglet with signs of congenital tremor and a healthy control piglet. A few multifocal, small vacuoles with variable shapes and sizes can be seen in equal numbers in the sick and healthy piglet. Scale bar: 200 µm. C and D: Cerebellar tissue from a piglet with signs of congenital tremor and a healthy control piglet stained with luxol fast blue stain and cresyl violet stain. No myelin loss or loss of nissl substances can be seen. Scale bar: 500 µm

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  Fig. 3. Oligodendrocytes from cerebellar tissue visualised by transmission electron microscopy. Transmission electron microscopic examination of cerebellar tissue from a piglet with signs of congenital tremor and a healthy control piglet. Mitochondria are marked with black arrows. In the healthy piglet, mitochondria are normal in size with a prominent crista. Numerous degenerated mitochondria, swollen with loss of crista, can be seen in the oligodendrocyte of the sick piglet

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  Fig. 4. Graph presenting the expression of STING and of IFN-α encoding genes. Significant upregulation, p < 0.05, of STING encoding gene in piglets PCR-positive for APPV and with signs of congenital tremor compared to the healthy control piglets. An up-regulation of IFN-α is indicated, however not statistically significant